By screening a pigeon genomic DNA library, we isolated a recombinant phage clone containing the alpha(A)-globin gene. The DNA sequence of the approximately 6kbp-long insert fragment of the phage clone was determined. The sequence suggested the existence of pigeon alpha(D)-globin gene located 3.1 kbp upstream from the alpha(A)-globin gene. The expression of the alpha(D)-globin in late embryo was also shown by the N-terminal amino-acid sequence of the intact globin chain. These results show that two adult alpha-globin genes, alpha(A) and alpha(D), exist in the pigeon genome, and the alpha(D)-globin is expressed at the late embryo stage. The stage-specific expression suggests the existence of regulatory elements and factors interacting to inhibit transcription at the adult stage.

译文

通过筛选鸽子基因组DNA文库,我们分离出含有 α (a)-珠蛋白基因的重组噬菌体克隆。确定了噬菌体克隆的约6kbp长的插入片段的DNA序列。该序列表明存在位于 α (A)-珠蛋白基因上游3.1 kbp的鸽子 α (D)-珠蛋白基因。完整珠蛋白链的N端氨基酸序列也显示了 α (D)-珠蛋白在晚期胚胎中的表达。这些结果表明,鸽子基因组中存在两个成年的 α-珠蛋白基因 α (A) 和 α (D),并且 α (D)-珠蛋白在胚胎晚期表达。阶段特异性表达表明存在调节元件和因子相互作用以抑制成年阶段的转录。

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