The width of retinal vessel images on fundus photographs was determined by projection micrometry and microdensitometry. These methods were evaluated on 12 retinal vessels using the negatives of 570-nm monochromatic fundus photographs. For projection micrometry, the intraobserver reproducibility of vessel width measurements was 1.6%-2.9%, depending upon the experience of the observer. Significant interobserver differences in the measured widths were demonstrated. For microdensitometry, three distinct measurement criteria were used. Significant differences in width as measured by the three criteria were found, but there were no interoperator differences for each criterion. The intraoperator reproducibility of vessel width measurements by microdensitometry was 2.1%-2.5%. Significant differences were found in the vessel widths determined by the micrometry and densitometry methods, and results obtained by micrometry are discussed in terms of edge-detection phenomena.

译文

通过投影显微法和显微密度法确定眼底照片上视网膜血管图像的宽度。使用570 nm单色眼底照片的底片在12条视网膜血管上评估了这些方法。对于投影显微测量,根据观察者的经验,血管宽度测量的观察者内重现性为1.6%-2.9%。证明了观察者之间在测量宽度上的显着差异。对于显微光密度测定法,使用了三种不同的测量标准。通过三个标准测得的宽度存在显着差异,但是每个标准都没有算子间差异。通过显微密度测定法测量血管宽度的操作者内部可重复性为2.1%-2.5%。在通过显微法和光密度法确定的血管宽度中发现了显着差异,并根据边缘检测现象讨论了通过显微法获得的结果。

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