Mayaro virus (alphavirus) infection of Aedes albopictus cells results in inhibition of cell protein synthesis and viral proteins are preferably synthesized. When infected cells are heat shocked, however, there is also an inhibition of viral protein synthesis, and there is preferential synthesis of heat shock proteins. Based on these observations, the distribution of Mayaro viral RNA in polysomes and the association of p34 (capsid protein) with ribosomal fractions of the cells under such conditions have been analyzed. During infection, the viral RNA is mainly observed in light polysomes (60% of total viral RNA in the cell) and also in heavy polysomes (13%). However, when infected cells are heat-shocked, the viral RNA is strongly mobilized from heavy polysomes to the light polysomes fraction and an enrichment in the unbound fraction can be noticed. The amount of p34 associated with the ribosomal fraction was also shown to be decreased in the heat shocked cells. These data lead to the suggestion that two mechanisms could be involved in the inhibition of Mayaro virus protein synthesis in response to heat shock(1) mobilization of Mayaro virus RNA from heavy to light polysomes; (2) a decrease in the amount of the p34 within the ribosomal fraction.

译文

白纹伊蚊细胞的Mayaro病毒 (α 病毒) 感染导致细胞蛋白合成的抑制,优选合成病毒蛋白。然而,当受感染的细胞被热休克时,病毒蛋白的合成也受到抑制,并且热休克蛋白的优先合成。基于这些观察结果,已经分析了在这种条件下,多核糖体中Mayaro病毒RNA的分布以及p34 (衣壳蛋白) 与细胞核糖体部分的关联。在感染期间,病毒RNA主要在轻多核糖体 (60% 细胞中总病毒RNA) 和重多核糖体 (13%) 中观察到。但是,当受感染的细胞受到热冲击时,病毒RNA会从重多核糖体强烈动员到轻多核糖体部分,并且可以注意到未结合部分的富集。在热休克细胞中,与核糖体级分相关的p34的量也显示出减少。这些数据表明,两种机制可能参与抑制马亚罗病毒蛋白合成,以响应热休克 (1) 马亚罗病毒RNA从重到轻多核糖体的动员; (2) 核糖体部分中p34的含量减少。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录