A study was conducted to determine whether following exposure of male mice to high temperatures, the ability of their spermatozoa to fertilise ova was reduced, especially during the period before the males became completely infertile. Male mice placed in a microclimate chamber at 36 degrees C for two periods, each of 12 h on successive days, were less able to fertilise control females in vivo when mated and, even in those females that became pregnant, litter size was reduced. However, these effects were associated with falls in testis weight and numbers of spermatozoa in the testis and epididymis. To determine whether the effect on fertility was a result of the decreased spermatozoa numbers, spermatozoa were collected from the epididymides of heated and control males. Equal numbers of motile spermatozoa from an unselected sample or those subjected to a swim-up procedure to separate those that were motile from the immotile ones in the sample were then mixed in vitro with oocytes from superovulated normal females. Similar numbers of spermatozoa from both control and heated males bound to the zona pellucida but smaller percentages of the oocytes were fertilised by spermatozoa from the heated males and fewer of these spermatozoa penetrated the ova. The effects were first seen 7 days after the heat exposure and became more obvious after 10 or 14 days.

译文

进行了一项研究,以确定雄性小鼠暴露于高温后,其精子受精卵子的能力是否降低,尤其是在雄性完全不育之前。在36摄氏度的小气候室中放置两个周期的雄性小鼠,每次连续12小时,交配时在体内受精对照雌性的能力较小,即使在怀孕的雌性中,产仔数也减少了。然而,这些影响与睾丸重量下降以及睾丸和附睾中精子数量下降有关。为了确定对生育力的影响是否是精子数量减少的结果,从加热和对照男性的附睾中收集了精子。然后将来自未选择样品的等量运动精子或经过游泳程序以将样品中运动的精子与未运动的精子分开的精子与超排卵正常雌性的卵母细胞在体外混合。与透明带结合的对照雄性和受热雄性的精子数量相似,但受热雄性的精子受精的卵母细胞百分比较小,而这些精子穿透卵子的数量较少。这种影响首先在暴露于热后7天出现,并在10或14天后变得更加明显。

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