Evaluation of genotoxicity of RISUG® - a vas based contraceptive, was carried out in the present study. Animals were allotted into groups of sham operated control, vas occlusion with RISUG (5-7 μl) for 360 days and reversal by DMSO (250-500 μl) and 5% NaHCO₃ (500 μl). Blood samples and testis were collected at 360 days of vas occlusion and 90 days of vas occlusion reversal for comet analysis. Hydrogen peroxide induced samples were used as positive control. Olive moment, tail length and percentage DNA in tail were recorded with minimum variation in all groups for both leukocytes and testis. When compared with positive control the variation was highly significant for both 20 μM and 50 μM H₂O₂ (p<0.001). It is concluded that vas occlusion with RISUG at the contraceptive dose regimen is not associated with genotoxicity in leukocytes or the testis of pre- and post-reversal rats.

译文

利苏格的遗传毒性评价®-在本研究中进行了基于vas的避孕药。将动物分为假手术对照组,用ristug (5-7μl) 阻塞vas 360天,并通过DMSO (250-500 μ l) 和5% nahco (500 μ l) 逆转。在360天的vas闭塞和90天的vas闭塞逆转时收集血样和睾丸以进行彗星分析。过氧化氢诱导的样品用作阳性对照。在所有组中,白细胞和睾丸均记录了橄榄时刻,尾巴长度和尾巴中的DNA百分比,变化最小。当与阳性对照相比时,变化对于20μm和50μmh 2 o 2都非常显着 (p<0.001)。结论是,在避孕剂量方案下,ristug阻塞vas与逆转前后大鼠的白细胞或睾丸的遗传毒性无关。

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