Proteins encoded by MADS-box genes are important transcription factors involved in the regulation of flowering plant growth and development. Currently, no systematic information exists regarding the MADS-box family in the important tropical fruit banana. Ninety-six MADS-box genes were identified from the banana (Pahang) A genome. Phylogenetic analysis indicated that Musa acuminata MCM1-AGAMOUS- DEFICIENS-SRF (MaMADS) could be divided into MIKCc, MIKC*, Mα/β and Mγ groups. MIKCc could be further divided into 11 subfamilies, which was further supported by conserved motif and gene structure analyses. Transcriptome analysis on the Feng Jiao (FJ) and BaXi Jiao (BX) banana cultivars revealed that MaMADS genes are differentially expressed in various organs, at different fruit development and ripening stages, indicating the involvement of these genes in fruit development and ripening processes. Interactive network analysis indicated that MaMADS24 and 49 not only interacted with MaMADS proteins themselves, but also interacted with hormone-response proteins, ethylene signal transduction and biosynthesis-related proteins, starch biosynthesis proteins and metabolism-related proteins. This systematic analysis identified candidate MaMADS genes related to fruit development and ripening for further functional characterization in plants, and also provided new insights into the transcriptional regulation of MaMADS genes, facilitating the future genetic manipulation of MADS-mediated fruit development and ripening.

译文

MADS-box基因编码的蛋白质是参与调节开花植物生长发育的重要转录因子。目前,尚无有关重要热带水果香蕉中MADS-box家族的系统信息。从香蕉 (Pahang) A基因组中鉴定出96个MADS-box基因。系统发育分析表明,尖锐湿疣MCM1-AGAMOUS-SRF (MaMADS) 可分为MIKCc,MIKC *,m α/β 和m γ 组。MIKCc可以进一步分为11个亚科,这得到了保守基序和基因结构分析的进一步支持。对Feng Jiao (FJ) 和BaXi Jiao (BX) 香蕉品种的转录组分析表明,MaMADS基因在不同的果实发育和成熟阶段在各个器官中差异表达,表明这些基因参与了果实的发育和成熟过程。交互网络分析表明,MaMADS24和49不仅与MaMADS蛋白本身相互作用,而且还与激素反应蛋白,乙烯信号转导和生物合成相关蛋白,淀粉生物合成蛋白和代谢相关蛋白相互作用。该系统分析确定了与果实发育和成熟相关的候选MaMADS基因,以进一步表征植物的功能,并为MaMADS基因的转录调控提供了新的见解,从而促进了MADS介导的果实发育和成熟的未来遗传操作。

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