Transgenic mice expressing high levels of the BclxL or Bcl2 proteins in the male germinal cells show a highly abnormal adult spermatogenesis accompanied by sterility. This appears to result from the prevention of an early and massive wave of apoptosis in the testis, which occurs among germinal cells during the first round of spermatogenesis. In contrast, sporadic apoptosis among spermatogonia, which occurs in normal adult testis, is not prevented in adult transgenic mice. The physiological early apoptotic wave in the testis is coincident, in timing and localization, with a temporary high expression of the apoptosis-promoting protein Bax, which disappears at sexual maturity. The critical role played by the intracellular balance, probably hormonally controlled, of the BclxL and Bax proteins (Bcl2 is apparently not expressed in normal mouse testis) in this early apoptotic wave is shown by the occurrence of a comparable testicular syndrome in mice defective in the bax gene. The apoptotic wave appears necessary for normal mature spermatogenesis to develop, probably because it maintains a critical cell number ratio between some germinal cell stages and Sertoli cells, whose normal functions and differentiation involve an elaborate network of communication.

译文

在雄性生发细胞中表达高水平BclxL或Bcl2蛋白的转基因小鼠显示出高度异常的成年精子发生并伴有不育。这似乎是由于防止了睾丸中早期大量的凋亡波,而这种凋亡波在第一轮精子发生期间发生在生发细胞中。相反,在成年转基因小鼠中,不能阻止正常成年睾丸中发生的精原细胞间的零星凋亡。睾丸中的生理性早期凋亡波在时间和定位上是重合的,与促凋亡蛋白Bax的暂时高表达,该蛋白在性成熟时消失。BclxL和Bax蛋白 (Bcl2显然在正常小鼠睾丸中未表达) 的细胞内平衡 (可能受激素控制) 在这种早期凋亡波中起关键作用,这表现为在小鼠中出现类似的睾丸综合征。bax基因。凋亡波似乎是正常成熟精子发生发展所必需的,可能是因为它在某些生发细胞阶段和支持细胞之间保持了临界的细胞数比,支持细胞的正常功能和分化涉及复杂的通讯网络。

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