This study examines the effect of age on oedema and brain swelling, and associated glial cell involvement on the size of the lesion in two models of permanent, focal cerebral ischaemia. Ischaemia was induced in male C57BL/Icrfat mice (4-6 and 26-31-month-old) by middle cerebral artery (MCA) occlusion using either electrocoagulation after craniotomy (MCA/craniotomy), or by an intraluminal filament through the carotid artery (MCA/icf). Twenty-four hours after inducing ischaemia, brain swelling and lesion size were measured in young and aged mice, and cerebral oedema by wet/dry brain weights. Histopathology and immunocytochemistry were performed on a separate set of perfusion fixed brains. The MCA/icf technique produced a significantly larger lesion than MCA/craniotomy in both age groups. The percentage of water taken into the brain was significantly greater after MCA/icf, with aged mice showing the greatest increase. When lesion size was corrected for brain swelling there was no age-related increase in the size of the lesion. The numbers of microglia and astroglia increased significantly in the parietal cortex of aged control animals, and there were qualitative differences in the glial response between the two stroke models. This study emphasizes the importance of age in models of permanent focal ischaemia, with oedema clearly being a significant factor. Differ-ences in the responsiveness of the glial cell population with age may be of fundamental importance in the progress of ischaemic brain damage.

译文

这项研究在两种永久性局灶性脑缺血模型中检查了年龄对水肿和脑肿胀以及相关的神经胶质细胞受累对病变大小的影响。在雄性C57BL/Icrfat小鼠 (4-6和26-31个月大) 中,通过开颅手术后的电凝 (MCA/开颅) 或通过腔内细丝通过大脑中动脉 (MCA) 闭塞诱发了缺血。颈动脉 (MCA/icf)。诱导缺血后24小时,在年轻和老年小鼠中测量脑肿胀和病变大小,并通过湿/干脑重量测量脑水肿。组织病理学和免疫细胞化学是在一组单独的灌注固定大脑上进行的。在两个年龄组中,MCA/icf技术产生的病变明显大于MCA/开颅手术。MCA/icf后,进入大脑的水百分比显着更高,其中衰老的小鼠显示出最大的增加。当将病变大小校正为脑肿胀时,病变大小没有与年龄相关的增加。老年对照动物的顶叶皮质中小胶质细胞和星形胶质细胞的数量显着增加,并且两种中风模型之间的神经胶质反应存在质的差异。这项研究强调了年龄在永久性局灶性缺血模型中的重要性,而水肿显然是一个重要因素。神经胶质细胞群体的反应性随年龄的变化可能对缺血性脑损伤的进展至关重要。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录