Anti-endothelial antibodies (AEA) have been described in patients with rheumatoid arthritis (RA) complicated by vasculitis. In this study we made use of an ELISA and immunoblot technique (IBT) to further characterize AEA of the IgG class in serum of patients with rheumatoid vasculitis (RV) and to investigate the relationship between the presence of IgG-AEA and vasculitis. IgG-AEA as measured by ELISA or IBT could be detected in the serum from 20 of the 23 (87%) RV patients, in 2 out of 13 (15%) patients with RA and in one of 15 healthy donors. The IBT revealed reactivity of IgG-AEA against a total of 12 bands of endothelial antigens ranging in size from 16 to 68 kD. IgG-AEA as measured by ELISA and IBT in serum samples of patients followed longitudinally were present more frequently and in higher titres in patients with active RV as compared to patients with vasculitis in remission. A significant correlation was found between the presence of clinical signs of vasculitis and serum IgG-AEA reactivity against an endothelial membrane antigen of 44 kD. These data show that the pattern of IgG-AEA reactivity in the serum of RV patients is heterogeneous and suggest that IgG-AEA against one particular antigen is involved in the pathogenesis of RV.

译文

抗内皮抗体 (AEA) 已被描述用于类风湿关节炎 (RA) 并发血管炎的患者。在这项研究中,我们使用ELISA和免疫印迹技术 (IBT) 进一步表征类风湿血管炎 (RV) 患者血清中IgG类的AEA,并研究IgG-AEA的存在与血管炎之间的关系。通过ELISA或IBT测量的IgG-AEA可以在23名 (87% 名) RV患者中的20名,13名 (15% 名) RA患者中的2名以及15名健康供体中的一名的血清中检测到。IBT显示IgG-AEA对总共12条大小为16至68 kD的内皮抗原带具有反应性。与血管炎缓解期患者相比,通过ELISA和IBT在纵向随访的患者血清样品中测量的IgG-AEA更频繁,并且在活动性RV患者中滴度更高。发现血管炎的临床体征与血清IgG-AEA对44 kd的内皮膜抗原的反应性之间存在显着相关性。这些数据表明,RV患者血清中IgG-AEA反应性模式是异质的,并表明针对一种特定抗原的IgG-AEA参与了RV的发病机理。

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