FSH is synthesized and secreted in multiple molecular forms with different oligosaccharide structures which are needed for full expression of biological activity. GnRH and sex steroids modulate oligosaccharide structure and composition. In the present study we have assessed the carbohydrate complexity and proportion of circulating FSH isoforms during puberty, aging and after androgen administration to pubertal anorchid boys. Preparative isoelectrofocusing and lectin chromatography were used to isolate FSH isoforms on the basis of charge and internal carbohydrate complexity. Differences in sialic acid content and a progressive increase of isoforms bearing highly branched oligosaccharides were found during puberty. Less acidic, more bioactive FSH isoforms, secreted at mid-puberty may modulate important maturational events in the Sertoli cell population. Androgen administration to pubertal anorchid boys favoured the secretion of this type of isoforms. In adult men, the predominance of FSH isoforms bearing complex type oligosaccharides remained unchanged until very advanced age. These results show that the predominance of FSH isoforms bearing fully processed oligosaccharides in circulation may contribute to the development and maintenance of seminiferous epithelium function in men.

译文

FSH以多种分子形式合成和分泌,具有不同的寡糖结构,是完全表达生物活性所必需的。GnRH和性类固醇调节寡糖的结构和组成。在本研究中,我们评估了青春期,衰老和对青春期anorchid男孩施用雄激素后的碳水化合物复杂性和循环FSH亚型的比例。根据电荷和内部碳水化合物的复杂性,使用制备性等电聚焦和凝集素色谱法分离FSH亚型。在青春期发现唾液酸含量的差异和带有高度分支寡糖的同工型的逐渐增加。青春期中期分泌的酸性较弱,生物活性较强的FSH亚型可能会调节Sertoli细胞群体中的重要成熟事件。对青春期男孩的雄激素给药有利于这种亚型的分泌。在成年男性中,带有复杂类型寡糖的FSH亚型的优势一直保持不变,直到非常高龄。这些结果表明,在循环中具有完全加工的寡糖的FSH亚型的优势可能有助于男性生精上皮功能的发展和维持。

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