Strongylocentrotus nudus is an edible sea urchin, mainly harvested in China. Correlation studies indicated that S. nudus with larger diameter have a prolonged marketing time and better palatability owing to their precocious gonads and extended maturation process. However, the molecular mechanism underlying this phenomenon is still unknown. Here, transcriptome sequencing was applied to study the ovaries of adult S. nudus with different shell diameters to explore the possible mechanism. In this study, four independent cDNA libraries were constructed, including two from the big size urchins and two from the small ones using a HiSeq™2500 platform. A total of 88,581 unigenes were acquired with a mean length of 1354bp, of which 66,331 (74.88%) unigenes could be annotated using six major publicly available databases. Comparative analysis revealed that 353 unigenes were differentially expressed (with log2(ratio)≥1, FDR≤0.001) between the two groups. Of these, 20 differentially expressed genes (DEGs) were selected to confirm the accuracy of RNA-seq data by quantitative real-time RT-PCR. Furthermore, gene ontology and KEGG pathway enrichment analyses were performed to find the putative genes and pathways related to ovarian maturity. Eight unigenes were identified as significant DEGs involved in reproduction related pathways; these included Mos, Cdc20, Rec8, YP30, cytochrome P450 2U1, ovoperoxidase, proteoliaisin, and rendezvin. Our research fills the gap in the studies on the S. nudus ovaries using transcriptome analysis.

译文

Strongylocentrotus nudus是一种可食用的海胆,主要在中国收获。相关研究表明,直径较大的S. nudus由于其早熟的性腺和成熟过程的延长,具有延长的销售时间和更好的适口性。然而,这种现象背后的分子机制仍然未知。在此,将转录组测序用于研究具有不同壳直径的成年S. nudus的卵巢,以探讨其可能的机制。在这项研究中,使用HiSeq构建了四个独立的cDNA文库,其中两个来自大海胆,两个来自小海胆。™2500平台。总共获得了88,581个单基因,平均长度为1354bp,其中66,331个 (74.88% 个) 单基因可以使用六个主要的公开数据库进行注释。比较分析显示,两组之间353单基因差异表达 (log2 (比率) ≥ 1,fdr ≤ 0.001)。其中,选择了20个差异表达基因 (deg),以通过定量实时rt-pcr确认RNA-seq数据的准确性。此外,还进行了基因本体论和KEGG途径富集分析,以找到与卵巢成熟度相关的推定基因和途径。八个单基因被鉴定为参与生殖相关途径的重要deg; 这些包括Mos,Cdc20,Rec8,YP30,细胞色素P450 2U1,卵巢过氧化物酶,蛋白毒素和rendezvin。我们的研究填补了使用转录组分析对卵巢S. nudus卵巢的研究的空白。

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