Rooibos leaves and fine stems (Aspalathus linearis; Fabaceae) are increasingly enjoyed as herbal tea, largely in fermented (oxidised) red-brown form, but also in unfermented (unoxidised) green form. Rooibos is rich in antioxidant polyphenols, with the dihydrochalcone, aspalathin, as a major active ingredient. We used Caenorhabditis elegans as model organism to investigate the effect of rooibos extracts against oxidative stress in vivo. In a high glucose environment, C. elegans treated with rooibos extract exhibited an extended lifespan. Furthermore, green rooibos was a more potent antioxidant than red rooibos, probably due to its substantially higher aspalathin content. In addition, rooibos decreased acute oxidative damage caused by the superoxide anion radical generator, juglone, with aspalathin playing a major role in improving the survival rate of C. elegans. Quantitative real-time PCR results demonstrated that aspalathin targets stress and ageing related genes, reducing the endogenous intracellular level of ROS. These findings suggest that rooibos increases stress resistance and promotes longevity under stress, probably mediated via a regulation of the DAF-16/FOXO insulin-like signalling pathway, supporting some of the health claims put forward for rooibos tea.

译文

Rooibos的叶子和细茎 (Aspalathus linearis; Fabaceae) 作为凉茶越来越受到欢迎,主要以发酵 (氧化) 的红棕色形式,但也以未发酵 (未氧化) 的绿色形式。Rooibos富含抗氧化剂多酚,其中二氢查耳酮,aspalathin是主要的活性成分。我们使用秀丽隐杆线虫作为模型生物,研究了rooibos提取物对体内氧化应激的影响。在高葡萄糖环境中,用rooibos提取物处理的秀丽隐杆线虫的寿命延长。此外,绿色rooibos是比红色rooibos更有效的抗氧化剂,这可能是由于其aspalathin含量高得多。此外,rooibos降低了由超氧阴离子自由基发生器juglone引起的急性氧化损伤,而aspalathin在提高秀丽隐杆线虫的存活率中起着重要作用。实时定量PCR结果表明,aspalathin靶向应激和衰老相关基因,降低了细胞内ROS的内源性水平。这些发现表明,rooibos可能通过调节DAF-16/FOXO胰岛素样信号通路介导,可以提高应激抵抗力并促进应激状态下的寿命,从而支持rooibos茶提出的一些健康要求。

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