IMPACT STATEMENT:The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.

译文

影响声明: 在各个发育阶段对食管癌进行分类对于确定患者的最佳治疗方案以及预后至关重要。食管癌分期的精度提高一直在寻求可以增强组织病理学技术的定量和分析成像方法。在这项工作中,我们使用非线性光学显微镜对食管鳞状细胞癌 (ESCC) 粘膜下层的双光子激发荧光 (TPEF) 和二次谐波产生 (SHG) 的固有信号进行了比率分析。TPEF/SHG和向前 (F)/向后 (B) SHG的盲测试被证明与组织学结论进行了比较。通过统计比较四个阶段的食管癌之间的敏感性和特异性的讨论。据我们所知,这是首次将这两种比率组合用于ESCC分期的研究。

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