Maternal malnutrition leads to the incidence of metabolic diseases in offspring. The purpose of this project was to examine whether maternal low chromium could disturb normal lipid metabolism in offspring, altering adipose cell differentiation and leading to the incidence of lipid metabolism diseases, including metabolic syndrome and obesity. Female C57BL mice were given a control diet (CD) or a low chromium diet (LCD) during the gestational and lactation periods. After weaning, offspring was fed with CD or LCD. The female offspring were assessed at 32 weeks of age. Fresh adipose samples from CD-CD group and LCD-CD group were collected. Genome mRNA were analysed using Affymetrix GeneChip Mouse Gene 2.0 ST Whole Transcript-based array. Differentially expressed genes (DEGs) were analysed based on gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis database. Maternal low chromium irreversibly increased offspring body weight, fat-pad weight, serum triglyceride (TG) and TNF-α. Eighty five genes increased and 109 genes reduced in the offspring adipose of the maternal low chromium group. According to KEGG pathway and String analyses, the PPAR signalling pathway may be the key controlled pathway related to the effect of maternal low chromium on female offspring. Maternal chromium status have long-term effects of lipid metabolism in female mice offspring. Normalizing offspring diet can not reverse these effects. The potential underlying mechanisms are the disturbance of the PPAR signalling pathway in adipose tissue.

译文

产妇营养不良导致后代代谢性疾病的发生。本课题的目的是研究母体低铬是否会干扰后代正常的脂质代谢,改变脂肪细胞的分化并导致脂质代谢疾病 (包括代谢综合征和肥胖) 的发生。在妊娠和哺乳期,对雌性C57BL小鼠进行对照饮食 (CD) 或低铬饮食 (LCD)。断奶后,用CD或LCD喂养后代。在32周龄时对雌性后代进行评估。收集cd-cd组和lcd-cd组的新鲜脂肪样品。使用Affymetrix GeneChip小鼠基因2.0 ST全转录物基阵列分析基因组mRNA。基于基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 途径分析数据库对差异表达基因 (DEGs) 进行分析。母体低铬不可逆地增加了后代体重,脂肪垫重量,血清甘油三酸酯 (TG) 和TNF-α。母体低铬组子代脂肪的85个基因增加,109个基因减少。根据KEGG途径和字符串分析,PPAR信号途径可能是与母体低铬对雌性后代影响有关的关键控制途径。母体铬状态对雌性小鼠后代的脂质代谢具有长期影响。使后代饮食正常化不能逆转这些影响。潜在的潜在机制是脂肪组织中PPAR信号通路的紊乱。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录