The morphology and the distribution of neurons expressing the NK1-receptor (NK1R) and the co-expression of gamma-aminobutyric acid (GABA) in these neurons were studied in the rat occipital cortex and in organotypic cultures (OTCs) derived from this structure. By employing immunohistochemistry, we demonstrate that the NK1R-expressing neurons are non-pyramidal neurons and co-express GABA. Some differences were noted between in vivo and OTCs. NK1R-expressing neurons in OTCs had larger somata and longer dendrites and the proportion stained with an anti-GABA-antibody (approximately 50%) was smaller than in vivo (90%). The preferential location of NK1R-expressing neurons in layers II/III and VI, seen in vivo is not present in OTCs where these neurons distribute rather homogeneously through layers II-VI. Our findings imply that in contrast to the cat and monkey, in the rat occipital cortex the effects of substance P are almost exclusively mediated via inhibitory interneurons.

译文

在大鼠枕叶皮层和衍生自该结构的器官型培养物 (otc) 中研究了表达NK1-receptor (NK1R) 的神经元的形态和分布以及 γ-氨基丁酸 (GABA) 的共表达。通过采用免疫组织化学,我们证明了NK1R-expressing神经元是非锥体神经元并且共表达GABA。注意到体内和otc之间的一些差异。Otc中的NK1R-expressing神经元具有较大的躯体和较长的树突,并且用抗GABA抗体染色的比例 (约50%) 小于体内 (90%)。在体内观察到的第II/III层和VI层中NK1R-expressing神经元的优先位置不存在于otc中,其中这些神经元相当均匀地分布在第ii-vi层中。我们的发现表明,与猫和猴子相反,在大鼠枕叶皮层中,p物质的作用几乎完全是通过抑制性中间神经元介导的。

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