Five isolates of coagulase-negative staphylococci were obtained from human urine, the gastrointestinal tract of squirrel monkeys, pig skin and from the environment. All key biochemical characteristics of the tested strains corresponded with the description of Staphylococcus xylosus species. However, partial 16S rRNA gene sequences obtained from analysed strains corresponded with those of Staphylococcus nepalensis reference strains, except for two strains which differed in one residue. Ribotyping with EcoRI and HindIII restriction enzymes, whole cell protein profile analysis performed by SDS-PAGE and SmaI macrorestriction analysis were used for more precise characterization and identification of the analysed strains. Obtained results showed that EcoRI and HindIII ribotyping and whole cell protein fingerprinting are suitable and reliable methods for the differentiation of S. nepalensis strains from the other novobiocin resistant staphylococci, whereas macrorestriction analysis was found to be a good tool for strain typing. The isolation of S. nepalensis is sporadic, and according to our best knowledge this study is the first report of the occurrence of this species in human clinical material as well as in other sources.

译文

从人尿,松鼠猴的胃肠道,猪皮和环境中获得了五种凝固酶阴性葡萄球菌分离株。测试菌株的所有关键生化特性均与木糖葡萄球菌种类的描述相对应。然而,从分析菌株获得的部分16S rRNA基因序列与尼泊尔葡萄球菌参考菌株的序列相对应,但两个菌株在一个残基上有所不同。使用EcoRI和HindIII限制性内切酶进行核糖分型,通过sdds-PAGE和SmaI大致伸缩分析进行的全细胞蛋白谱分析用于更精确的表征和鉴定所分析的菌株。获得的结果表明,EcoRI和HindIII核素分型和全细胞蛋白指纹图谱是从其他耐新霉素葡萄球菌中分化出尼泊尔链球菌菌株的合适且可靠的方法,而大致伸缩分析被发现是菌株分型的良好工具。尼泊尔芽孢杆菌隔离是零星的,据我们所知,这项研究是该物种在人类临床材料以及其他来源中出现的第一份报告。

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