Nonhost resistance as a durable and broad-spectrum defence strategy is of great potential for agricultural applications. We have previously isolated a cDNA showing homology with genes encoding bZIP transcription factors from tomato leaf mould pathogen Cladosporium fulvum. Upon expression, the cDNA results in necrosis in C. fulvum host tomato and nonhost tobacco plants and is thus named CfHNNI1 (for C . f ulvum host and nonhost plant necrosis inducer 1). In the present study we report the induction of necrosis in a variety of nonhost plant species belonging to three families by the transient in planta expression of CfHNNI1 using virus-based vectors. Additionally, transient expression of CfHNNI1 also induced expression of the HR marker gene LeHSR203 and greatly reduced the accumulation of recombinant Potato virus X. Stable CfHNNI1 transgenic tobacco plants were generated in which the expression of CfHNNI1 is under the control of the pathogen-inducible hsr203J promoter. When infected with the oomycetes pathogen Phytophthora parasitica var. nicotianae, these transgenic plants manifested enhanced expression of CfHNNI1 and subsequent accumulation of CfHNNI1 protein, resulting in high expression of the HSR203J and PR genes, and strong resistance to the pathogen. The CfHNNI1 transgenic plants also exhibited induced resistance to Pseudomonas syringae pv. tabaci and Tobacco mosaic virus. Furthermore, CfHNNI1 was highly expressed and the protein was translocated into plant cells during the incompatible interactions between C. fulvum and host and nonhost plants. Our results demonstrate that CfHNNI1 is a potential general elicitor of hypersensitive response and nonhost resistance.

译文

非宿主抗性作为一种持久的广谱防御策略,在农业应用中具有巨大的潜力。我们以前已经分离出了一个cDNA,该cDNA显示出与番茄叶片霉菌病原体fulvum的bZIP转录因子编码基因的同源性。表达后,该cDNA在黄连寄主番茄和非寄主烟草植物中导致坏死,因此被命名为CfHNNI1 (对于黄连寄主和非寄主植物坏死诱导剂1)。在本研究中,我们报告了使用基于病毒的载体通过CfHNNI1在植物中的瞬时表达诱导了属于三个科的多种非宿主植物物种的坏死。此外,CfHNNI1的瞬时表达还诱导了HR标记基因LeHSR203的表达,并大大减少了重组马铃薯X病毒的积累。产生了稳定的CfHNNI1转基因烟草植物,其中CfHNNI1的表达在病原体诱导的hsr203J启动子的控制下。当感染卵菌病原体疫霉菌时。烟草,这些转基因植物表现出增强的CfHNNI1表达和随后的CfHNNI1蛋白积累,导致HSR203J和PR基因的高表达,以及对病原体的强抗性。CfHNNI1转基因植物还表现出对丁香假单胞菌pv的诱导抗性。烟粉虱和烟草花叶病毒。此外,在C. fulvum与宿主和非宿主植物之间的不相容相互作用期间,CfHNNI1高度表达,并且蛋白质被转运到植物细胞中。我们的结果表明,CfHNNI1是超敏反应和非宿主抗性的潜在一般诱发因素。

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