Five different hydrophobin-encoding cDNA clones from Cladosporium fulvum were isolated from cDNA libraries, made from nutrient-depleted mycelium. One cDNA clone was identical to the previously isolated hydrophobin HCf-1. The other clones were named HCf-2, -3, -4 and -5. HCf-1, -2, -3 and -4 show a high degree of identity, and are predicted to encode class I hydrophobins. HCf-5 encodes a class II hydrophobin. The expression patterns of these hydrophobins at various stages of development, and in liquid media lacking either carbon or nitrogen, or both, showed clear differences. All hydrophobins were more strongly expressed during sporulation than before, with HCf-4 and -5 showing the highest increase. Expression of HCf genes in infected plants was also higher at 16 days than at 10 days after infection. The expression of HCf-5 in sporulating mycelium was much lower in planta than in vitro. All HCf genes were upregulated under conditions of nutrient deprivation. HCf-1, -2, -3 and -4 showed highest levels of transcription in medium lacking both carbon and nitrogen. Expression of HCf-5 was highest in medium lacking nitrogen but containing carbon. HCf-1 was generally the most abundant hydrophobin. The introduction of multiple copies of HCf-1, which caused co-suppression of the endogenous HCf-1 gene, was shown to affect the expression of HCf-2, -3 and -4 also. Expression of HCf-4 was suppressed, but expression of HCf-2 and -3 was upregulated. Expression of HCf-5 was not changed.

译文

从营养耗尽的菌丝体制成的cDNA文库中分离出五个不同的疏水蛋白编码cDNA克隆。一个cDNA克隆与先前分离的疏水蛋白HCf-1相同。其他克隆被命名为HCf-2、-3、-4和-5。HCf-1、-2、-3和-4显示出高度的同一性,并且预测编码I类疏水蛋白。HCf-5编码II类疏水蛋白。这些疏水蛋白在发育的各个阶段以及在缺乏碳或氮或两者的液体培养基中的表达模式显示出明显的差异。所有疏水蛋白在孢子形成过程中比以前更强烈地表达,其中HCf-4和-5显示出最高的增加。感染后16天,感染植物中HCf基因的表达也高于感染后10天。植物中HCf-5在孢子菌丝体中的表达远低于体外。在营养剥夺条件下,所有HCf基因均上调。HCf-1,-2,-3和-4在缺乏碳和氮的培养基中显示出最高水平的转录。在缺乏氮但含碳的培养基中,HCf-5的表达最高。HCf-1通常是最丰富的疏水蛋白。引入多个拷贝的HCf-1引起内源HCf-1基因的共抑制,也会影响HCf-2,-3和-4的表达。HCf-4的表达被抑制,但HCf-2和-3的表达被上调。HCf-5的表达没有改变。

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