The role of different growth media and chemical enhancer on synthesis of secondary metabolites Cladosporium resinae (NRL-6437) was investigated for their in vitro biological activities. Cladosporium resinae (NRL-6437) were grown in various nutrient media (Czapeak-dox Broth (CB), Czapeak Yeast-extract Broth (CYB), Yeast Extract Sucrose (YES), Potato Dextrose Broth (PDB) and Czapeak-dox (supplemented with glucose and starch) Broth (CGSB) for the production of metabolites. Two chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) were also used for the expression of silent genes for secondary metabolite production. Our results indicated that among different media, Czapeak yeast extract broth produced more secondary metabolites. Application of 15mM of both modifiers was effective for the expressions of silent genes resulting in an increased metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane were also tested for their biological activity. The secondary metabolites revealed varying degrees of growth inhibitions of the tested organisms. Similarly, these metabolites were also active against brine shrimps and Lemna.

译文

研究了不同生长培养基和化学增强剂对次生代谢产物枝孢菌 (NRL-6437) 的体外生物活性的作用。在各种营养培养基 (Czapeak-dox肉汤 (CB),Czapeak酵母提取物肉汤 (CYB),酵母提取物蔗糖 (是) 中生长reinae (NRL-6437),马铃薯葡萄糖肉汤 (PDB) 和Czapeak-dox (补充葡萄糖和淀粉) 肉汤 (CGSB) 用于生产代谢物。两种化学表观遗传修饰剂 (sueroyl-anilide异羟肟酸 (SAHA) 和5-氮杂胞苷 (5-AZA) 也用于表达沉默基因以用于次级代谢产物生产。我们的结果表明,在不同的媒体中,czapeak酵母提取物肉汤产生了更多的次级代谢产物。应用15毫米的两种修饰剂对沉默基因的表达是有效的,从而增加了代谢产物的产生。还测试了在乙酸乙酯中提取并在正己烷中分馏的次级代谢产物的生物活性。次级代谢产物揭示了不同程度的生长抑制作用被测试的生物。同样,这些代谢物对卤虾和Lemna也具有活性。

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