Linoleic acid hydroperoxide (HPOD), substrate of hydroperoxide lyase, an enzyme of the lipoxygenase pathway, can be transformed into many aromatic compounds, the so-called "green notes". The presence of linoleic acid hydroperoxide in the culture medium of Yarrowia lipolytica, the yeast expressing the cloned hydroperoxide lyase of green bell pepper, undoubtedly exerted an inhibition on the growth and a toxic effect with 90% of yeast cells died after 120 min of exposition in 100 mM HPOD solution. The increase in cell membrane fluidity evaluated by measuring fluorescence generalized polarization with the increasing concentration of HPOD in the medium confirmed the fluidizing action of HPOD on yeast membrane. In addition, we determined by infrared spectroscopy measurement that this compound rapidly diffused into model phospholipids [1, 2-Dimyristoyl-D54-sn-Glycero-3-Phosphocholine (DMPC-D54)] bilayer, modifying their general physical state and their phase transition. In the presence of various concentrations of HPOD, the phase transition of DMPC-D54 occurred with an increase of both the corresponding wave number shift and the temperature range but the phase transition temperature was not modified. These results show that the toxic effects of HPOD on the yeast Yarrowia lipolytica may be initially linked to a strong interaction of this compound with the cell membrane phospholipids and components.

译文

亚油酸氢过氧化物 (HPOD),氢过氧化物裂解酶的底物,脂氧合酶途径的一种酶,可以转化为许多芳香族化合物,即所谓的 “绿色笔记”。表达青椒克隆的氢过氧化物裂解酶的酵母解脂酵母的培养基中存在亚油酸氢过氧化物,无疑对生长产生了抑制作用,并且在100 mM HPOD溶液中暴露120分钟后,酵母细胞90% 死亡。通过测量荧光广义极化评估的细胞膜流动性随培养基中HPOD浓度的增加而增加,证实了HPOD对酵母膜的流化作用。此外,我们通过红外光谱测量确定该化合物迅速扩散到模型磷脂 [1,2-Dimyristoyl-D54-sn-Glycero-3-Phosphocholine (DMPC-D54)] 双层中,从而改变了它们的一般物理状态和它们的相变。在存在各种浓度的HPOD的情况下,DMPC-D54的相变随着相应的波数偏移和温度范围的增加而发生,但相变温度没有改变。这些结果表明,HPOD对酵母解脂酵母的毒性作用最初可能与该化合物与细胞膜磷脂和组分的强相互作用有关。

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