The mode of action of Vibrio vulnificus haemolysin (VVH) on mast cells from the peritoneal cavity of the rat was examined. VVH induced histamine release, and damage to the mast cells, in a dose-dependent fashion. When 1 microgram of VVH was added to c. 10(5) mast cells at 37 degrees C, histamine release was observed after a lag period of 5-10 s, and was complete within 5 min. The action was temperature-dependent, and was not induced at 4 degrees C. Disodium cromoglycate, a membrane stabiliser for mast cells, inhibited the histamine release significantly, but the effect was not dose-dependent. Moreover, leakage of lactate dehydrogenase from VVH-treated mast cells was observed. These results suggest that VVH acts on the cell membrane of mast cells and is cytolytic.

译文

检查了创伤弧菌溶血素 (VVH) 对大鼠腹膜腔肥大细胞的作用方式。VVH以剂量依赖性方式诱导组胺释放并损害肥大细胞。当在37 ℃ 下将1微克VVH加入c. 10(5) 肥大细胞时,在5-10 s的滞后期后观察到组胺释放,并在5分钟内完成。该作用与温度有关,在4 ℃ 下未诱导。色甘氨酸二钠是肥大细胞的膜稳定剂,可显着抑制组胺的释放,但其作用不是剂量依赖性的。此外,观察到乳酸脱氢酶从VVH处理的肥大细胞中泄漏。这些结果表明,VVH作用于肥大细胞的细胞膜并具有溶细胞性。

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