Interferon alpha (IFN-alpha) induces cytogenetic responses of variable degree in patients with CML. We sought to establish the relationship between BCR-ABL transcript numbers measured by competitive two-step reverse transcription polymerase chain reaction (RT-PCR) and cytogenetic status in CML patients treated with IFN-alpha. All 398 samples from 163 patients investigated by RT-PCR were positive for BCR-ABL transcripts. In order to standardize results for variability in RNA and cDNA quality, we quantified total ABL transcripts in each sample as internal control. The BCR-ABL/ABL ratios correlated with the cytogenetic results. Quantitative nested PCR allowed the detection of residual BCR-ABL transcripts in all complete cytogenetic responders on IFN-alpha. We conclude that competitive PCR with internal controls is a reliable method for monitoring patients on IFN-alpha and reduces the need for repeated marrow investigations.

译文

干扰素 α (IFN-α) 诱导CML患者不同程度的细胞遗传学反应。我们试图建立通过竞争性两步逆转录聚合酶链反应 (rt-pcr) 测量的bcr-abl转录数与接受IFN-α 治疗的CML患者的细胞遗传学状态之间的关系。通过rt-pcr调查的来自163名患者的所有398样品的bcr-abl转录物均为阳性。为了标准化RNA和cDNA质量变异性的结果,我们将每个样品中的总ABL转录本量化为内部对照。Bcr-abl/ABL比值与细胞遗传学结果相关。定量巢式PCR可以检测到IFN-α 上所有完整的细胞遗传学反应中的残留BCR-ABL转录本。我们得出的结论是,带有内部对照的竞争性PCR是一种可靠的方法,用于监测患者的IFN-α,并减少了重复进行骨髓检查的需要。

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