The cellular distribution of muscarinic acetylcholine receptor protein in the frontal cortex of Alzheimer (AD) patients, age-matched and middle-aged controls was assessed quantitatively by means of immunohistochemistry using the monoclonal antibody M35. As shown previously in biopsy cortices, mainly layer II/III and V pyramidal neurons were immunolabeled. Neither distribution nor numbers of labeled cells displayed significant differences between the groups investigated. This is in accordance with the results of ligand binding studies that mostly failed to reveal different binding characteristics in AD compared to controls. Muscarinic and nicotinic receptor proteins have been shown to be colocalized in many cholinoceptive pyramidal neurons. Since nicotinic receptors--in contrast to muscarinic receptor proteins--are severely reduced in AD, this indicates a selective impairment of nicotinic receptor expression and not a significant death of cholinoceptive neurons per se.

译文

通过使用单克隆抗体m35的免疫组织化学方法定量评估了阿尔茨海默病 (AD) 患者,年龄匹配和中年对照的额叶皮层中毒蕈碱型乙酰胆碱受体蛋白的细胞分布。如先前在活检皮层中所示,主要对II/III层和V锥体神经元进行了免疫标记。在所研究的组之间,标记细胞的分布和数量均未显示出显着差异。这与配体结合研究的结果一致,该研究大多未能揭示与对照组相比在AD中的不同结合特性。毒蕈碱和烟碱受体蛋白已显示在许多胆碱感受性锥体神经元中共定位。由于烟碱受体-与毒蕈碱受体蛋白相反-在AD中严重减少,这表明烟碱受体表达的选择性损害,而不是胆碱感受性神经元本身的显着死亡。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录