Coxiella burnetii causes Q fever, an important zoonotic disease, and exposure is mainly associated with inhalation of contaminated aerosols. In South America, no systematic studies have been carried out. In Chile, the only official record of Q fever has been an outbreak of occupational context occurring in 1998 with eight confirmed human cases, all workers in the Agriculture and Livestock Service. Recently, in 2017 a Q fever outbreak was reported from dairy farm workers in two regions in southern Chile. This study determined the presence of C. burnetii in bulk tank milk samples from dairy farms obtained during this outbreak. A duplex real time quantitative PCR assay with primers and probes targeting two different gene sequences, IS1111 and com1, was used for diagnosis. C. burnetii was detected in 2 of 105 samples analyzed (2.1%). These results pose a potential public health risk as the milk from these farms was sold to the local human population. This is the first report on detecting C. burnetii in raw tank milk samples in Chile.

译文

布氏柯氏菌引起Q发热,这是一种重要的人畜共患病,接触主要与吸入受污染的气溶胶有关。在南美洲,没有进行系统的研究。在智利,Q发热的唯一官方记录是职业背景的爆发,1998年有8例确诊的人类病例,所有都是农业和畜牧业部门的工人。最近,智利南部两个地区的奶牛场工人2017年爆发Q发热。这项研究确定了在这次爆发期间从奶牛场获得的散装罐装牛奶样品中是否存在C. burnetii。使用靶向两个不同基因序列IS1111和com1的引物和探针的双链实时定量PCR检测来诊断。在分析的105个样品中的2个中检测到C. burnetii (2.1%)。这些结果构成了潜在的公共健康风险,因为这些农场的牛奶被出售给当地居民。这是智利首次在生罐牛奶样品中检测到burnetii C. burnetii的报告。

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