A dynamic thermal time model (DTTM) has been developed to predict cold-hardiness in dormant grapevine buds, which uses daily mean temperatures as the only input variable. However, it has been recently reported that cold-hardiness in grapevine buds depends not only on low temperatures (LTs), but also on the content of the plant phytohormone abscisic acid (ABA). An important parameter in the DTTM is the ecodormancy boundary (EDB), which represents the chilling degree days (DDc) required for the transition of the buds from endo to ecodormancy. In this study, the bud cold-hardiness of grapevines was measured by the low-temperature exotherm (LTE) in the subtropical Elqui and in the temperate Maipo valleys of Chile, and the values fitted to the DTTM. Moreover, the ABA content was determined in dormant buds throughout the dormant season. The results demonstrated that the DTTM worked better in the Maipo than in the Elqui valley, and that the content of ABA in the buds was higher in the vines grown in the Elqui valley. On the other hand, the optimized EDB parameter value varied from year to year when it was estimated as chilling accumulated, but when it was estimated as time, it remained constant in the Maipo valley. Based on the results, we conclude the following: (a) the proper functioning of the DTTM requires that the ABA content profile in the dormant buds should be kept constant from year to year, (b) the highest content of ABA in the buds collected in the Elqui valley is probably due to abiotic stresses, (c) the EDB parameter corresponds to the accumulated cold until before the buds begin their deacclimation process and not until they release from endodormancy, (d) the release of the buds from endodormancy does not depend on the accumulated cold, but on the elapsed time.