BACKGROUND:The genes cagA and vacA encode H pylori virulence factors.

AIM:To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms.

MATERIAL AND METHODS:We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA genotyping was done using polymerase chain reactions.

RESULTS:The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1.

CONCLUSIONS:In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2.

译文

背景 : 基因cagA和vacA编码幽门螺杆菌毒力因子。
目标 : 为了在从上消化道症状患者中分离出的幽门螺杆菌菌株中对这些基因进行基因型。
材料和方法 : 我们研究了50例接受上消化道内窥镜检查且培养阳性的患者幽门螺杆菌。使用聚合酶链反应检测cagA和vacA基因分型。
结果 : 在19个样品中检测到cagA基因 (38%)。分别在16个样品中检测到vacA基因的信号序列s1和s2 (32%)。在6个样品中同时扩增s1和s2,在9个样品中未检测到。9个样品中vacA的中间区域为m1,29个样品中m2,12个样品中m1和m2同时扩增。在16个样品 (32%) 中,检测到多于一种类型的信号序列或中间区域。在仅检测到vacA基因型的患者中,有15例为s2/m2,7例为s1/m1,4例为s1/m2,1例为s2/m1。
结论 : 在这些患者中,以cagA- H pylori菌株感染为主,s1或s2菌株的感染率相似,主要的内侧区域为m2。

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