Genetic factors are important for developing primary and subsequent malignancies in children. This study investigated the role of genetic factors involved in DNA-repair. Designed as a feasibility study, it addressed the possibility of obtaining samples for genetic analyses from former patients through the German Childhood Cancer Registry. Testing feasibility was as important as the biological question itself. We analyzed the expression of DNA-repair genes in untreated primary fibroblasts of 20 individuals with a second neoplasm compared to 20 matched single neoplasm cases using customized cDNA microarrays (1344 gene sequences, about 800 genes). Matching was by first neoplasm, age, and year of first diagnosis. Forty-six percent of the 52 contacted second neoplasm cases and 18% of the 132 single neoplasm patients participated in the study. The DNA-repair gene results show small differences in the basal gene expression of FTH1 and CDKN1A. To our knowledge, this is the first study using gene expression arrays in untreated primary fibroblasts regarding second neoplasms after a childhood neoplasm. We were able to recruit childhood cancer patients for genetic analyses long after diagnosis. The biological importance of the differences in the DNA-repair gene expression has to be elucidated yet.

译文

遗传因素对于发展儿童原发性和继发恶性肿瘤很重要。这项研究调查了参与DNA修复的遗传因素的作用。作为一项可行性研究,它解决了通过德国儿童癌症登记处从以前的患者那里获得用于基因分析的样本的可能性。测试可行性与生物学问题本身一样重要。我们使用定制的cDNA微阵列 (1344个基因序列,约800个基因) 分析了20个具有第二个肿瘤的个体的未处理的原代成纤维细胞中DNA修复基因的表达,与20个匹配的单个肿瘤病例相比。根据首次肿瘤,年龄和首次诊断的年份进行匹配。52例接触第二肿瘤病例中的6% 例和132例单一肿瘤患者中的18% 例参加了研究。DNA修复基因结果显示FTH1和CDKN1A的基础基因表达差异很小。据我们所知,这是第一项使用基因表达阵列在未经治疗的原发性成纤维细胞中进行的关于儿童肿瘤后第二次肿瘤的研究。我们能够在诊断后很长时间招募儿童癌症患者进行基因分析。必须阐明DNA修复基因表达差异的生物学重要性。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录