The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.

译文

两种人类蛋白质Ki-1/57和CGI-55具有高度相似的氨基酸序列,但其功能未知。我们通过酵母双杂交筛选对它们进行了分析,发现它们与人染色质重塑因子CHD-3 (色解旋酶-DNA结合结构域蛋白-3) 的C端区域相互作用。CGI-55和CHD-3的相互作用可以通过Sf9昆虫细胞的免疫共沉淀在体外和体内得到证实。作图显示,CGI-55通过其N和C末端的两个区域与CHD-3相互作用。CGI-55和Ki-1/57 mrna在肌肉、结肠和肾脏中表达最高。CGI55-GFP融合蛋白位于HeLa细胞的细胞质,细胞核和核周区域。这些数据表明,CGI-55和Ki-1/57可能参与核功能,如染色质的重塑。

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