OBJECTIVE:To examine the relationship between infection with Mycoplasma and the development of rheumatoid arthritis (RA) and juvenile rheumatoid arthritis (JRA).

METHODS:Immunoblotting of patient synovial fluid and sera on detergent-phase membrane protein extracts of various Mycoplasma species was carried out to learn whether patients exhibited serologic evidence of previous exposure to mycoplasmas. Moreover, an ultrasensitive polymerase chain reaction (PCR) method was developed for assessing whether Mycoplasma DNA could be detected in synovial fluid from patients and controls.

RESULTS:Immunoblotting provided serologic evidence of previous Mycoplasma exposure in patients and controls. The genus-specific PCR detected known human Mycoplasma species and could reliably detect <5 copies of Mycoplasma hominis, Mycoplasma fermentans, or a molecular mimic control in synovial fluid. Repeat testing revealed no evidence of Mycoplasma DNA in patient synovial samples.

CONCLUSION:This study provided serologic evidence suggesting that, while previous exposure to Mycoplasma was common, there was no detectable persistence of Mycoplasma DNA in the synovial fluid or tissue of patients with RA or JRA.

译文

目的 : 检查支原体感染与类风湿关节炎 (RA) 和幼年类风湿性关节炎 (JRA) 的发展之间的关系。
方法 : 对各种支原体的去污剂相膜蛋白提取物进行了患者滑液和血清的免疫印迹,以了解患者是否表现出先前暴露于支原体的血清学证据。此外,开发了一种超灵敏的聚合酶链反应 (PCR) 方法,用于评估是否可以在患者和对照组的滑液中检测到支原体DNA。
结果 : 免疫印迹提供了患者和对照组先前暴露于支原体的血清学证据。属特异性PCR检测到已知的人类支原体物种,并且可以可靠地检测滑液中 <5个拷贝的人支原体,发酵支原体或分子模拟对照。重复测试显示患者滑膜样本中没有支原体DNA的证据。
结论 : 这项研究提供了血清学证据,表明尽管先前暴露于支原体很常见,RA或JRA患者的滑液或组织中没有可检测到支原体DNA的持久性。

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