In human cerebral cortex slices noradrenaline, isoproterenol (a beta-adrenergic agonist), dopamine, apomorphine (a dopaminergic agonist), and serotonin stimulated cyclic AMP formation: noradrenaline greater than or equal to isoproterenol greater than dopamine = apomorphine = serotonin. Clonidine (and alpha-adrenergic agonist) was ineffective in stimulating cyclic AMP formation in temporal cortex slices. The stimulatory effect of noradrenaline and isoproterenol was blocked by propranolol (a beta-adrenergic blocker) but not by phentolamine (an alpha-adrenergic blocker). Pimozide (a selective dopaminergic antagonist) inhibited the increase of cyclic AMP formation induced by dopamine or apomorphine but not that induced by noradrenaline, isoproterenol, or serotonin. Neither propranolol or phentolamine had any effect on dopamine- or serotonin-stimulated cyclic AMP formation. Chlorpromazine blocked the increase of cyclic AMP formation induced by noradrenaline, dopamine or serotonin, while cyproheptadine, a putative central serotonergic antagonist, was ineffective. These observations suggest that there may be at least two monoamine-sensitive adenylate cyclases in human cerebral cortex which have the characteristics of a beta-adrenergic and a dopaminergic receptor, respectively, and also possibly a serotonergic receptor.

译文

在人大脑皮层切片中去甲肾上腺素,异丙肾上腺素 (β-肾上腺素能激动剂),多巴胺,阿扑吗啡 (多巴胺能激动剂) 和5-羟色胺刺激的环状AMP形成: 去甲肾上腺素大于或等于异丙肾上腺素大于多巴胺 = 阿扑吗啡 = 5-羟色胺。可乐定 (和 α-肾上腺素能激动剂) 在刺激颞叶皮质切片中的环状AMP形成方面无效。去甲肾上腺素和异丙肾上腺素的刺激作用被普萘洛尔 (一种 β-肾上腺素能阻滞剂) 阻断,而酚妥拉明 (一种 α-肾上腺素能阻滞剂) 则不能。Pimozide (一种选择性多巴胺能拮抗剂) 抑制多巴胺或阿扑吗啡诱导的环AMP形成的增加,但不能抑制去甲肾上腺素,异丙肾上腺素或5-羟色胺诱导的环AMP形成。普萘洛尔或酚妥拉明均不会对多巴胺或5-羟色胺刺激的环状AMP形成产生任何影响。氯丙嗪阻止了由去甲肾上腺素,多巴胺或5-羟色胺引起的环状AMP形成的增加,而被认为是中枢5-羟色胺能拮抗剂的赛庚啶无效。这些观察结果表明,人大脑皮层中可能至少存在两种单胺敏感的腺苷酸环化酶,它们分别具有 β-肾上腺素能受体和多巴胺能受体的特征,也可能具有血清素能受体的特征。

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