This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function.

译文

这项研究旨在评估以下假设: 将复制缺陷型重组腺病毒载体施用到呼吸道上皮表面可用于将重组蛋白以足够量递送到体循环,以引起适合的全身反应。重组蛋白。我们向具有免疫活性的Balb/c小鼠的呼吸道上皮施用AdCMV.TPO-一种含有编码人血小板生成素 (TPO) cDNA的表达盒的腺病毒载体。在接下来的一周中,血清人TPO水平升高,血小板水平增加了六倍以上,并且骨髓中巨核细胞增多。该策略可能是非胃肠外给药多种治疗性重组蛋白的有用方法,例如与凝血,内分泌功能和骨髓功能有关的蛋白。

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