Various bacteria are associated with ectomycorrhizal roots, which are symbiotic complexes formed between plant roots and fungi. Among these associated bacteria, actinomycetes have received attention for their ubiquity and diverse roles in forest ecosystems. Here, to examine the association of actinomycetes with ectomycorrhizal root tips, we compared the bacterial and actinomycete communities on the surface and inside of root tips of coastal Japanese black pine (Pinus thunbergii) colonized by the fungus Cenococcum geophilum. Next-generation sequences of 16S rDNA of bacteria communities using the Ion Torrent Personal Genome Machine showed that the number of bacterial classes in the surface of C. geophilum ECM roots was significantly higher than that in non-ECM roots. The bacterial community structure of surface, inside, and non-ECM roots was significantly discriminated each other. For an isolation method, a total of 762 and 335 actinomycete isolates were obtained from the surface and inside of the roots, respectively. In addition, the isolation ratio of actinomycetes in these root tips varied depending on the age of the tree and the season. Identification of the isolates based on partial 16S rDNA sequencing revealed that the isolates belonged to nine genera of the order Actinomycetales. On the surface of the roots, most of the isolates belonged to genus Streptomyces (90.4%); inside of the roots, most of the isolates belonged to genus Actinoallomurus (40.0%), which is a relatively new taxon. Our results suggest that actinomycetes as well as bacteria are ubiquitously associated with C. geophilum ectomycorrhizal roots of P. thunbergii, although their communities can vary either surface or inside of individual root tips.

译文

:各种细菌与根外生根有关,根外生根是植物根和真菌之间形成的共生复合物。在这些相关细菌中,放线菌因其在森林生态系统中的普遍存在和不同作用而受到关注。在这里,为了检查放线菌与根外菌根尖的关联,我们比较了由真菌Cenococcum geophilum繁殖的日本黑松(Pinus thunbergii)根尖的表面和内部的细菌和放线菌群落。使用离子洪流型个人基因组机的细菌群落的16S rDNA的下一代序列显示,嗜地衣芽孢杆菌ECM根表面的细菌种类数量显着高于非ECM根。表面,内部和非ECM根的细菌群落结构彼此之间有明显区别。对于分离方法,分别从根的表面和内部获得了总共762和335放线菌的分离株。另外,这些根尖中放线菌的分离率根据树的年龄和季节而变化。基于部分16S rDNA测序的分离物的鉴定表明,分离物属于放线菌纲的9个属。在根的表面上,大多数分离物属于链霉菌属(Streptomyces)(90.4%)。在根部内部,大多数分离物属于放线菌属(Actinoallomurus)(40.0%),这是一个相对较新的分类群。我们的结果表明,放线菌以及细菌普遍与雷氏假单胞菌的地衣芽孢杆菌外生菌根相关,尽管它们的群落可以在单个根尖的表面或内部变化。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录