The asexual ectomycorrhizal fungus Cenococcum geophilum has a wide geographic range in diverse forest ecosystems. Although its genetic diversity has been documented at a stand or regional scale, knowledge of spatial genetic structure is limited. We studied the genetic diversity and spatial structure of C. geophilum in eight Japanese coastal pine forests with a maximum geographic range of 1364 km. A total of 225 samples were subjected to phylogenetic analysis based on the glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) followed by microsatellite analysis with five loci. The phylogenetic analysis based on GAPDH resolved three groups with most isolates falling into one dominant lineage. Microsatellite analyses generated 104 multilocus genotypes in the overall populations. We detected significant genetic variation within populations and genetic clusters indicating that high genetic diversity may be maintained by possible recombination processes at a stand scale. Although no spatial autocorrelation was detected at a stand scale, the relationship between genetic and geographic distances among the populations was significant, suggesting a pattern of isolation by distance. These results indicate that cryptic recombination events at a local scale and unknown migration events at both stand and regional scales influence spatial distribution and genetic structure of C. geophilum in coastal pine forests of Japan.

译文

:无性外生菌根真菌Cenococcum geophilum在不同的森林生态系统中具有广泛的地理范围。尽管已在林分或区域范围内记录了其遗传多样性,但对空间遗传结构的了解有限。我们研究了八种日本沿海松林中地衣梭菌的遗传多样性和空间结构,其最大地理范围为1364公里。总共225个样品根据甘油醛3-磷酸脱氢酶基因(GAPDH)进行了系统发育分析,然后通过五个位点进行了微卫星分析。基于GAPDH的系统发育分析可分为三类,大多数分离物属于一个优势谱系。微卫星分析在总种群中产生了104个多基因座基因型。我们在种群和遗传簇中检测到显着的遗传变异,表明在标准规模上可能通过可能的重组过程维持了较高的遗传多样性。尽管在林分尺度上未检测到空间自相关,但种群之间的遗传距离与地理距离之间的关系很重要,这表明了按距离隔离的模式。这些结果表明,在日本沿海松林中,局部范围内的神秘重组事件以及林分和区域范围内的未知迁移事件都影响了地衣梭菌的空间分布和遗传结构。

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