Aqueous suspension of magnetite nanoparticles with primary diameter of 10 nm were intratracheally administered into rat lungs. In 24 h, cells were isolated from bronchoalveolar lavage and examined under a transmission electron microscope. Alveolar macrophages demonstrated ability to actively uptake single nanoparticles and small aggregates composed of such particles, which then formed larger conglomerates inside fused phagosomes. Some of these mature phagosomes shed the membrane and free nanoparticles closely interacted with nuclear membrane and with cristae and mitochondrial membranes thereby inflicting pronounced damage to these intracellular structures. The loss of primary lysosomes can be viewed as indirect evidence attesting to the role played by diffusion of lysosomal hydrolytic enzymes in the final destruction of the alveolar macrophages provoked by nanoparticles.

译文

:将气管内施用初级直径为10 nm的磁铁矿纳米颗粒的水悬浮液注入大鼠肺中。在24小时内,从支气管肺泡灌洗液中分离细胞,并在透射电子显微镜下检查。肺泡巨噬细胞显示出能够主动摄取单个纳米颗粒和由此类颗粒组成的小聚集体的能力,然后这些小聚集体会在融合的吞噬体内形成较大的团块。这些成熟的吞噬体中的一些脱落了膜,游离的纳米颗粒与核膜以及cr和线粒体膜紧密相互作用,从而对这些细胞内结构造成了明显的破坏。初级溶酶体的损失可被视为间接证据,证明溶酶体水解酶的扩散在纳米颗粒引起的肺泡巨噬细胞的最终破坏中发挥了作用。

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