The cold acclimation process in plants is primarily regulated through the signal transduction pathways that lead to the induction and enhancement of expression of different sets of Cor/Lea genes. Winter wheat 'Mironovskaya 808' (M808) exhibited a much higher level of freezing tolerance than spring wheat 'Chinese Spring' (CS), and the difference became clearer after the long-term cold acclimation. To understand the molecular basis of this cultivar difference, we isolated two CBF/DREB1 homologs, Wcbf2, which are the candidate gene for a transcription factor of the Cor/Lea genes. Expression of the Wcbf2 gene was induced rapidly by low temperature (LT) and drought but not by abscisic acid (ABA). The gene expression was temporal and at least twice up-regulated by LT. The first up-regulation occurred within 1-4 h, which might correspond to the rapid response to LT, while the second up-regulation occurred during 2-3 weeks of cold acclimation. After the second up-regulation, the amount of Wcbf2 transcript greatly decreased in CS, while it increased again in M808 after 4 weeks until 9 weeks (end of the test period). The maintenance of this high level of the Wcbf2 transcript might represent the long-term effect of cold acclimation. The activation of Cor/Lea genes followed the accumulation of Wcbf2 transcript suggested direct involvement of the Wcbf2 gene in the induction and enhancement of the Cor/Lea gene expression. The cultivar difference in freezing tolerance developed during different stages of cold acclimation can be at least partly explained by the differential and coordinated regulation of the predicted Cor/Lea gene signal transduction pathway that is mediated by the CBF/DREB1 transcription factors in common wheat.

译文

:植物的冷驯化过程主要通过信号转导途径来调节,该信号转导途径导致不同Cor / Lea基因集的表达的诱导和增强。冬小麦“ Mironovskaya 808”(M808)的耐寒性要比春小麦“中国春”(CS)高得多,经过长期的冷驯化,差异变得更加明显。为了了解此品种差异的分子基础,我们分离了两个CBF / DREB1同源物Wcbf2,它们是Cor / Lea基因转录因子的候选基因。 Wcbf2基因的表达是由低温(LT)和干旱迅速诱导的,而不是由脱落酸(ABA)诱导的。基因表达是暂时的,并且至少两次被LT上调。第一次上调发生在1-4小时内,这可能与对LT的快速反应相对应,而第二次上调发生在冷适应的2-3周内。第二次上调后,CS中的Wcbf2转录物数量大大减少,而在4周后直到9周(测试期结束),其在M808中再次增加。保持这种高水平的Wcbf2转录本可能代表冷驯化的长期影响。 Wcbf2转录物的积累跟随着Cor / Lea基因的激活,表明Wcbf2基因直接参与了Cor / Lea基因表达的诱导和增强。在冷驯化的不同阶段中形成的耐寒性品种差异至少可以部分通过普通小麦的CBF / DREB1转录因子介导的预测Cor / Lea基因信号转导途径的差异和协调调节来解释。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录