The epidemiology of emerging antibiotic resistance genes in Asia is inadequately defined and studies within the major pools of transmissible genes such as integron gene cassettes are important. One hundred and twenty-two non-repetitive Acinetobacter spp. isolates were obtained from inpatients of a major hospital in South China. Fifty-three of these isolates contained class 1 integrons, and there is evidence of horizontal gene transfer between unrelated clones. The common pool of gene cassettes was dominated by four cassette arrays: arr3-aacA4 (24 isolates of several unrelated strains); aacC1-orfP-orfQ-aadA1a (11 isolates, probably all the same strain); aacA4-catB8-aadA1 (2 isolates); and dfrVII (1 isolate). We developed a simple restriction fragment length polymorphism (RFLP)-based identification of these and other cassettes reported in China, using readily available enzymes, to facilitate further studies of this type.

译文

亚洲新兴抗生素抗性基因流行病学定义不充分,在整合子基因盒等主要可传播基因库中的研究很重要。122种非重复不动杆菌属。分离株是从华南一家大型医院的住院患者中获得的。这些分离株中有53个包含1类整合子,并且有证据表明不相关克隆之间存在水平基因转移。基因盒的共同库主要由四个盒阵列组成: arr3-aacA4 (几个无关菌株的24个分离株); aacC1-orfP-orfQ-aadA1a (11个分离株,可能都是相同的菌株); aacA4-catB8-aadA1 (2个分离株); 和dfrVII (1个分离株)。我们开发了一种基于简单限制性片段长度多态性 (RFLP) 的中国报道的这些和其他盒的鉴定,使用现成的酶,以促进对这种类型的进一步研究。

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