Five carbapenem-resistant Acinetobacter baumannii isolates, collected from the United Arab Emirates in 2006, were investigated to identify the mechanism(s) responsible for carbapenem resistance. Genotyping was performed by pulsed-field gel electrophoresis, and the location of the bla(OXA-23) gene was determined by using the endonuclease I CeuI technique and mating-out assays. The four isolates in which the bla(OXA-23) gene was located on the chromosome within a Tn2006 composite transposon were clonally related. The single non-clonally related isolate harboured the bla(OXA-23) gene on a 70-kb transferable plasmid. This study reports on the dissemination of OXA-23-producing A. baumannii isolates in the Middle East.

译文

研究了从阿拉伯联合酋长国2006年收集的五种耐碳青霉烯类鲍曼不动杆菌分离株,以确定引起碳青霉烯类耐药的机制。通过脉冲场凝胶电泳进行基因分型,并通过使用内切核酸酶I ceeu技术和交配试验确定bla(OXA-23) 基因的位置。其中bla(OXA-23) 基因位于Tn2006复合转座子内染色体上的四个分离株是克隆相关的。单个非克隆相关分离物将bla(OXA-23) 基因保存在70kb可转移质粒上。本研究报告了OXA-23-producing鲍曼不动杆菌分离株在中东的传播情况。

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