Semitelechelic HPMA (N-(2-hydroxypropyl)methacrylamide) copolymers possessing a single terminal lipophilic triphenylphosphonium (TPP) cation and fluorescent labels were synthesized to determine how the attached cation affected cellular uptake and intracellular trafficking. In vitro mitochondrial uptake fluorescence quenching assays using isolated mouse liver mitochondria indicated that only lower molecular weight (<5 kDa) BODIPY FL-labeled TPP-semitelechelic HPMA copolymers exhibited significant organelle localization or uptake. In vitro cellular uptake and intracellular trafficking was evaluated using cultured human ovarian carcinoma cells. Cells incubated with all types of TPP copolymers used in the study appeared to internalize the polymer by endocytosis only, and all of the internalized copolymer was confined to the lysosomal compartment after 24 h. Endocytotic uptake of the TPP-HPMA copolymer conjugates was rapid, suggesting that they were internalized by adsorptive endocytosis, rather than fluid-phase pinocytosis. Low-molecular weight (<5 kDa) and high-molecular weight (>5 kDa) semitelechelic copolymers, microinjected into cultured cells indicated that the TPP moiety did not significantly localize the polymers to mitochondria.

译文

合成具有单个末端亲脂性三苯基phosph(TPP)阳离子和荧光标记的半螯合HPMA(N-(2-羟丙基)甲基丙烯酰胺)共聚物,以确定连接的阳离子如何影响细胞摄取和细胞内运输。使用分离的小鼠肝线粒体进行的体外线粒体摄取荧光猝灭测定表明,只有较低分子量(<5 kDa)的BODIPY FL标记的TPP-半螯合HPMA共聚物显示出明显的细胞器定位或摄取。使用培养的人卵巢癌细胞评估了体外细胞摄取和细胞内运输。与研究中使用的所有类型的TPP共聚物一起孵育的细胞似乎仅通过内吞作用使聚合物内在化,并且所有内在化的共聚物在24小时后均被限制在溶酶体区室中。 TPP-HPMA共聚物共轭物的胞吞摄取很快,这表明它们是通过吸附性内吞作用而不是液相的胞吞作用而被内在化的。显微注射到培养细胞中的低分子量(<5 kDa)和高分子量(> 5 kDa)半遥望共聚物表明TPP部分并未将聚合物显着定位于线粒体。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录