The putative key codon (Lys-76 in sensitive parasites and Thr-76 in resistant parasites) of the novel candidate gene for chloroquine resistance, Plasmodium falciparum chloroquine resistance transporter (pfcrt), was determined by polymerase chain reaction-restriction fragment length polymorphism from 111 Cameroonian isolates and was compared with in vivo and in vitro responses to chloroquine. The key codon was significantly associated (P< .001) with responses in vivo (92% sensitivity and 76% specificity) and in vitro (97% sensitivity and 81% specificity). Some discordant results were due to multiclonal infections. The high, but not perfect, correlation between the pfcrt polymorphism and the phenotype implies that a single point mutation in codon 76 of the pfcrt gene is the major, but possibly not the sole, determinant for chloroquine resistance.

译文

:通过聚合酶链反应-限制性片段长度多态性分析,确定了新的候选氯喹抗性候选基因,恶性疟原虫氯喹抗性转运蛋白(pfcrt)的推定关键密码子(敏感寄生虫中的Lys-76和抗性寄生虫中的Thr-76)。并与111种喀麦隆分离物进行了比较,并与体内和体外对氯喹的反应进行了比较。关键密码子与体内(92%敏感性和76%特异性)和体外(97%敏感性和81%特异性)应答显着相关(P <.001)。一些不一致的结果是由于多克隆感染引起的。 pfcrt多态性与表型之间的高度相关但不是完美的相关性意味着pfcrt基因第76位密码子的单点突变是决定氯喹抗性的主要因素,但不是唯一的决定因素。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录