Three new spirostanol pentaglycosides embracing beta-D-apiofuranose were isolated from the fresh underground parts of Chlorophytum comosum together with four known saponins. The structures of new compounds were determined by spectroscopic data, including two-dimensional NMR, and partial acid-catalysed hydrolysis to be (25R)-5 alpha-spirostane-2 alpha,3 beta-diol 3-O-[O-beta-D-glucopyranosyl- (1-->2)-O-[O-beta-D-apiofuranosyl-(1-->4)-beta-D-glucopyranosyl-(1 -->3)]-O- beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranoside], (25R)-3 beta-hydroxy-5 alpha-spirostan-12-one (hecogenin) 3-O-[O-beta-D-glucopyranosyl-(1-->2)-O-[O-beta-D-apiofuranosyl-(1- ->4)- beta-D-xylopyranosyl-(1-->3)]-O-beta-D-glucopyranosyl-(1-->4)-beta-D- galactopyranoside] and hecogenin 3-O-[O-beta-D-glucopyranosyl-(1-->2)-O-[O-beta-D- apiofuranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->3)]-O-beta-D-gluc opyranosyl- (1-->4)-beta-D-galactopyranoside], respectively. The isolated saponins were examined for inhibitory activity using 12-O-tetradecanoylphorbor-13-acetate-stimulated 32P-incorporation into phospholipids of HeLa cells as the primary screening test to identify new antitumour-promoter compounds.

译文

:从新鲜的绿藻地下部分中分离了三种含有β-D-apiofuranose的新螺甾烷醇五糖苷和四种已知的皂苷。通过光谱数据(包括二维NMR)和部分酸催化的水解,确定新化合物的结构为(25R)-5α-spirostane-2α,3β-二醇3-O- [O-β- D-吡喃葡萄糖基-(1-> 2)-O- [O-β-D-apiofuranosyl-(1-> 4)-beta-D-吡喃葡萄糖基-(1-> 3)]-O- beta- D-吡喃吡喃糖基-((1-> 4)-β-D-吡喃半乳糖苷],(25R)-3β-羟基-5α-spirostan-12-one(hecogenin)3-O- [O-beta-D-吡喃葡萄糖基-(1-> 2)-O- [O-beta-D-apiofuranosyl-(1--> 4)-beta-D-吡喃吡喃糖基-(1-> 3)]-O-beta-D-吡喃葡萄糖基-(1-> 4)-β-D-吡喃半乳糖苷]和hecogenin 3-O- [O-β-D-吡喃葡萄糖基-(1-> 2)-O- [O-β-D-apiofuranosyl- (1→4)-β-D-吡喃葡萄糖基-(1→3)]-O-β-D-葡糖吡喃糖基-(1→4)-β-D-吡喃半乳糖苷]。作为主要的筛选试验,使用12-O-十四烷酰phorbor-13-乙酸盐刺激的32P掺入HeLa细胞磷脂中来检查分离的皂苷的抑制活性,以鉴定新的抗肿瘤促进剂化合物。

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