Calligonum polygonoides is locally called as Phog which belongs to the Polygonaceae family. It is traditionally used as an antifungal. The methanolic extract, hexane extract, ethyl acetate extract and aqueous extract were screened against Candida albicans and Aspergillus niger in seven concentrations, that is, 1.8, 2.9, 6.5, 12.6, 25, 50 and 75 µg/mL/disc. Calligonum polygonoides showed significant activity against Candida albicans as the observed minimum inhibitory concentration (MIC) is 6.5 µg/mL for methanolic extract, 9.8 µg/mL for ethyl acetate extract, whereas aqueous and hexane extracts showed no activity. Calligonum polygonoides did not show any significant activity against Aspergillus niger.