This laboratory has previously demonstrated that shortening of the cell body of a heterotrich ciliate, Blepharisma japonicum, could be induced as a step-down photophobic response. Here, we examined the structure and contractility of the myonemes in detergent-extracted cell models and in isolated cortical fragments. Ultrastructural observation showed that the myoneme was connected to the basal ends of the posterior kinetosomes and constructed a systematic network as a whole. Shortening of the cell model was induced by > 10(-4) M Ca(2+), while the rounded cell model did not re-elongate even when it was washed in a calcium-free solution either with or without addition of ATP. Fluffy fibrils, which were tentatively identified as aggregated bundles of the myonemes, were isolated with the kinetosomal complex and showed calcium-dependent and ATP-independent contraction. The minimum concentration of Ca(2+) required for inducing contraction was at the level of 10(-6) M. These results suggest that the cell body shortening in Blepharisma is caused by the Ca(2+)-dependent contraction of the myonemal network.

译文

:该实验室先前已证明杂多纤毛纤毛虫(Blepharisma japonicum)的细胞体缩短可以作为逐步降低的憎光反应而被诱导。在这里,我们检查了去污剂提取的细胞模型和分离的皮层碎片中的模因的结构和收缩性。超微结构观察表明,肌球蛋白与后运动体的基端相连,并构成了一个整体的系统网络。细胞模型的缩短是由> 10(-4)M Ca(2)引起的,而圆形细胞模型即使在无钙溶液中添加或不添加ATP洗涤也不会重新伸长。蓬松的原纤维被初步确定为肌源蛋白的聚集束,与运动体复合物分离并显示出钙依赖性和ATP依赖性收缩。诱导收缩所需的Ca(2)的最低浓度为10(-6)M。这些结果表明,血吸虫病中的细胞体缩短是由肌层网络的Ca(2)依赖性收缩引起的。

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