OBJECTIVE:Evolving evidence of anti-inflammatory effects is observed in patients with rheumatoid arthritis or ulcerative colitis following periodic adsorptive granulocyte and monocyte (GM) apheresis with a column containing cellulose acetate (CA) beads as apheresis carriers. This study was undertaken to obtain insights into mechanisms of anti-inflammatory actions of adsorptive GM apheresis with CA beads.
METHODS:In a series of in-vitro experiments, we investigated the effects of plasma proteins and the leucocytes beta2 integrin (CD18) on granulocyte adsorption to CA beads.
RESULTS:Granulocyte adsorption to CA beads required plasma IgG, the complement C3 and was inhibited by an antibody to leucocytes CD18. Further, hepatocyte growth factor (HGF) and interleukin-1 receptor antagonist (IL-1ra) which have strong anti-inflammatory actions were released by granulocytes that adhered to CA beads.
CONCLUSIONS:Plasma IgG, C3 derived complement activation fragments and leucocytes CD18 are involved in granulocyte adhesion to CA beads and hence the release of HGF and IL-1ra.
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【Adhesion dependent release of hepatocyte growth factor and interleukin-1 receptor antagonist from human blood granulocytes and monocytes: evidence for the involvement of plasma IgG, complement C3 and beta2 integrin.].
【黏附依赖性从人血粒细胞和单核细胞释放肝细胞生长因子和白介素-1受体拮抗剂：血浆IgG，补体C3和β2整联蛋白参与的证据。】
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DOI：10.1007/s00011-004-1253-5文章类型：杂志文章
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目的：观察到类风湿性关节炎或溃疡性结肠炎患者在定期吸收性粒细胞和单核细胞（GM）血液采血后，使用醋酸纤维素（CA）珠作为血液采血载体的色谱柱，具有抗炎作用的不断发展的证据。进行这项研究是为了获得对带有CA珠粒的吸附性GM血液单采的抗炎作用机制的见解。
方法：在一系列体外实验中，我们研究了血浆蛋白和白细胞β2整合素（CD18）对粒细胞吸附CA珠的影响。
结果：粒细胞吸附到CA珠上需要血浆IgG，补体C3，并被白细胞CD18抗体抑制。此外，附着于CA珠的粒细胞释放出具有强抗炎作用的肝细胞生长因子（HGF）和白介素-1受体拮抗剂（IL-1ra）。
结论：血浆IgG，C3衍生的补体激活片段和白细胞CD18参与粒细胞与CA珠的粘附，并因此释放HGF和IL-1ra。
方法：在一系列体外实验中，我们研究了血浆蛋白和白细胞β2整合素（CD18）对粒细胞吸附CA珠的影响。
结果：粒细胞吸附到CA珠上需要血浆IgG，补体C3，并被白细胞CD18抗体抑制。此外，附着于CA珠的粒细胞释放出具有强抗炎作用的肝细胞生长因子（HGF）和白介素-1受体拮抗剂（IL-1ra）。
结论：血浆IgG，C3衍生的补体激活片段和白细胞CD18参与粒细胞与CA珠的粘附，并因此释放HGF和IL-1ra。
