Triton-fractionated photosystem-I particles poised at -625 mV, where the two bound iron-sulfur proteins are reduced, have been studied by optical and electron paramagnetic resonance spectroscopies from 293 to 5 K. At 5-9 K, these particles exhibit two decay components with lifetimes of 1.3 and 130 msec in the laser pulse-induced absorption and electron paramagnetic resonance signal changes. Spectral properties of the 130-msec decay component reflect the charge separation between P-700 and some iron-sulfur center having a broad optical absorbance in the 400- to 550-nm region and a previously reported electron paramagnetic resonance signal with g = 1.78, 1.88, and 2.08. Spectral properties of the 1-msec decay component indicate photoinduced charge separation between P-700 and a chlorophyll a dimer having absorption bands at 420, 450, and 700 nm. It is assumed that these two acceptors participate in the electron transfer from P-700(*) to the bound iron-sulfur proteins.

译文

已通过光学和电子顺磁共振光谱从293 K到5 K研究了位于-625 mV的Triton分级的光系统I颗粒,其中两个结合的铁硫蛋白被还原。在5-9 K时,这些粒子在激光脉冲诱导的吸收和电子顺磁共振信号变化中表现出两种寿命为1.3和130毫秒的衰变分量。130毫秒衰减分量的光谱特性反映了P-700与某些铁硫中心之间的电荷分离,该铁硫中心在400至550纳米区域具有宽的光吸收,并且先前报道的电子顺磁共振信号具有g = 1.78、1.88和2.08。1毫秒衰减组分的光谱特性指示P-700和叶绿素a二聚体之间的光诱导电荷分离,叶绿素a二聚体具有在420、450和700 nm处的吸收带。假定这两个受体参与了从P-700(*) 到结合的铁硫蛋白的电子转移。

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