Shewanella woodyi cultures were used to correlate bioluminescence intensity with changes in the electrochemical potential of a saltwater medium using soluble electron acceptors. A relationship between the concentration of NaNO3 or CoCl2 to bioluminescence intensity was confirmed using aerobic cultures of S. woodyi at 20°C with glucose as the sole carbon source. In general, increasing the concentration of nitrate or Co(II) reduced the bioluminescence per cell, with complete luminescence being repressed at ≥5 mM nitrate and ≥0.5 mM Co(II). Results from cell viability fluorescent staining concluded that increasing the concentration of Co(II) or nitrate did not affect the overall viability of the cells when compared with cultures lacking Co(II) or nitrate. These data show that potentials of <0.2 V vs Normal Hydrogen Electrode (NHE) repress the luminescence from the cells, but the exact mechanism is unclear. Our results indicated that the luminescence intensity from S. woodyi could be systematically reduced using these two soluble electron acceptors, making S. woodyi a potential model bacterium for whole-cell luminescence bioelectrochemical sensor applications.

译文

使用Shewanella woodyi培养物将生物发光强度与使用可溶性电子受体的盐水介质的电化学电势的变化相关联。使用以葡萄糖为唯一碳源的woodyi S. woodyi的有氧培养物,在20 °C下确认了NaNO3或CoCl2的浓度与生物发光强度之间的关系。通常,增加硝酸盐或Co(II) 的浓度会降低每个细胞的生物发光,在 ≥ 5毫米硝酸盐和 ≥ 0.5 mM Co(II) 处抑制完全发光。细胞活力荧光染色的结果得出结论,与缺乏Co(II) 或硝酸盐的培养物相比,增加Co(II) 或硝酸盐的浓度不会影响细胞的整体活力。这些数据表明,相对于正常氢电极 (NHE) 的 <0.2 V的电势抑制了细胞的发光,但确切的机制尚不清楚。我们的结果表明,使用这两个可溶性电子受体可以系统地降低S. woodyi的发光强度,从而使S. woodyi成为全细胞发光生物电化学传感器应用的潜在模型细菌。

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