Objective: To determine the effect of electro-acupuncture (EA) treatment on serum levels of interferon-γ (IFN-γ) in rats with 7,12-dimethylbenz(α)anthracene (DMBA)-induced breast tumors. Methods: Twenty five female Wistar rats were divided randomly into 5 groups: normal group (N; neither DMBA-induced nor treated with EA); control group (C; DMBA-induced only); EA 3 days : (DMBA-induced + EA for 3 days); EA 5 days: (DMBA-induced + EA for 5 days); EA 10 days: (DMBA-induced + EA for 10 days) group. Animals were acclimatized from day 1 to day 7. Subcutaneus injections of DMBA 10mg/kg BW was administered every second day, from days 7 to 35. Acupuncture was performed every second day from day 42. Rats were sacrificed on the second day after the last acupuncture, breast tumors excised and stained histological sections were analysed by light microscopy. At sacrifice, blood was extracted from the heart for measurement of serum IFN-γ by ELISA. Results: All of the DMBA-induced rats developed tumors. Electro-acupuncture significantly increased IFN-γ levels in DMBA induced rats, when compared to control group. Conclusions: Our findings suggest that EA significantly increases IFN-γ levels in DMBA-induced breast tumors.

译文

:目的:确定电针治疗对血清中干扰素-γ(IFN-γ)水平的影响
在患有7,12-二甲基苯并(α)蒽(DMBA)诱导的乳腺肿瘤的大鼠中。方法:25名女性Wistar
将大鼠随机分为5组:正常组(N;既不是DMBA诱导的,也不是用EA治疗的)。控制
组(C;仅DMBA诱导); EA 3天:(DMBA诱导的EA 3天); EA 5天:(DMBA诱导的EA
5天); EA 10天:(DMBA诱导的EA 10天)组。从第1天到第7天使动物适应环境。
从第二天到第7天至第35天,皮下注射DMBA 10mg / kg体重。
从第42天起每隔第二天进行一次。最后一次针刺后第二天将大鼠处死,
通过光学显微镜分析切除的肿瘤和染色的组织学切片。牺牲时,血液被抽出
从心脏通过ELISA测定血清IFN-γ。结果:所有DMBA诱导的大鼠均出现肿瘤。
与对照组相比,电针显着增加了DMBA诱导的大鼠的IFN-γ水平。
结论:我们的发现表明,EA可以显着增加DMBA诱导的乳腺肿瘤中的IFN-γ水平。

+1
+2
100研值 100研值 ¥99课程
检索文献一次
下载文献一次

去下载>

成功解锁2个技能,为你点赞

《SCI写作十大必备语法》
解决你的SCI语法难题!

技能熟练度+1

视频课《玩转文献检索》
让你成为检索达人!

恭喜完成新手挑战

手机微信扫一扫,添加好友领取

免费领《Endnote文献管理工具+教程》

微信扫码, 免费领取

手机登录

获取验证码
登录