High-fat-feeding in rats has been reported to enhance stress reactions, as assessed by elevation of blood glucose and corticosterone levels. This study was designed to investigate the relationship between changes in blood glucose and hypothalamic neuronal noradrenaline activity (HNNA), as indexed by the ratio of dihydroxyphenylethyleneglycol (DHPG) to noradrenaline (NA), following physiological stress in high-fat-fed rats. Two groups of adult male Wistar rats were fed isocaloric diets high in fat (59% of calories) or starch (70% of calories). After 3 weeks each of these groups was further subdivided into (a) control, (b) 2 min ambient temperature (20 degrees C) swim or (c) 2 min swim in ice-cold water. Animals were decapitated 20 min after commencing the swim; trunk blood and a sample of medial basal hypothalamus were obtained. Computerized gas chromatography/mass spectrometry was used to measure hypothalamic DHPG and NA concentrations. There were no differences between fat- and starch-fed rats in basal levels of serum glucose, insulin or corticosterone and no differences in DHPG, NA or DHPG/NA ratio. Compared to starch-fed rats, ambient swim stress in the fat-fed group produced significantly larger serum glucose (P less than 0.01), serum corticosterone (P less than 0.05), DHPG (P less than 0.05) and DHPG/NA (P less than 0.01) responses. Following cold swim stress similar differences between fat- and starch-fed animals were observed. In addition, serum insulin was found to be significantly suppressed in the fat-fed group (P less than 0.05) following cold swim.(ABSTRACT TRUNCATED AT 250 WORDS)

译文

:据报道,高血糖喂养可增强大鼠的应激反应,通过血糖和皮质酮水平的升高来评估。本研究旨在研究高脂饮食大鼠生理应激后血糖变化与下丘脑神经元去甲肾上腺素活性(HNNA)之间的关系,该关系以二羟苯基乙二醇(DHPG)与去甲肾上腺素(NA)之比表示。两组成年雄性Wistar大鼠以高热量(占热量的59%)或淀粉(占热量的70%)的等热量饮食喂养。 3周后,将这些组中的每组进一步细分为(a)对照,(b)2分钟环境温度(20摄氏度)游泳或(c)在冰冷水中游泳2分钟。开始游泳后20分钟,将动物斩首。获得干血和内侧下丘脑基底样品。使用计算机气相色谱/质谱法测量下丘脑的DHPG和NA浓度。脂肪和淀粉喂养的大鼠之间的基础血糖,胰岛素或皮质酮水平无差异,DHPG,NA或DHPG / NA比也无差异。与淀粉喂养的大鼠相比,脂肪喂养组的周围游泳压力产生显着更大的血清葡萄糖(P小于0.01),血清皮质酮(P小于0.05),DHPG(P小于0.05)和DHPG / NA(P小于0.01)的响应。冷泳后,在脂肪和淀粉喂养的动物之间观察到相似的差异。另外,冷饮后脂肪喂养组的血清胰岛素被显着抑制(P小于0.05)。(摘要截断为250个字)

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