Here, a novel mycovirus, Botryosphaeria dothidea mitovirus 1 (BdMV1), was isolated from a phytopathogenic fungus, Botryosphaeria dothidea, and its molecular characteristics were determined. BdMV1 has a genome of 2,667 nt that contains a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 727 amino acids with a molecular mass of 81.64 kDa. BLASTp analysis revealed that the RdRp domain of BdMV1 has 39.59% and 39.18% sequence identity to Plasmopara viticola associated mitovirus 43 and Setosphaeria turcica mitovirus 1, respectively. Phylogenetic analysis further suggested that BdMV1 is a new member of the genus Mitovirus within the family Mitoviridae. To the best of our knowledge, this is the first report of a mitovirus in B. dothidea.

译文

:在这里,从一种植物病原性真菌Botryosphaeria dothidea中分离出了一种新型的分枝杆菌Bothosphaeria dothidea线粒体病毒1(BdMV1),并确定了其分子特性。 BdMV1具有2,667 nt的基因组,其中包含使用真菌线粒体遗传密码的单个大开放阅读框(ORF)。 ORF编码一个727个氨基酸的RNA依赖性RNA聚合酶(RdRp),分子量为81.64 kDa。 BLASTp分析显示,BdMV1的RdRp结构域分别与葡萄膜疟原虫相关的线粒体病毒43和粘粒线粒体线粒体病毒1具有39.59%和39.18%的序列同一性。系统发育分析进一步表明,BdMV1是线粒体病毒科中线粒体病毒属的新成员。据我们所知,这是首次在轮状芽胞杆菌中出现线粒体病毒的报道。

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