BACKGROUND:Lysin motif-containing proteins (LYP), which act as pattern-recognition receptors, play central roles in growth, node formation, and responses to biotic stresses. The sequence of Chinese white pear genome (cv. 'Dangshansuli') along with the seven other species of Rosaceae has already been reported. Although, in these fruit crops, there is still a lack of clarity regarding the LYP family genes and their evolutionary history. RESULTS:In the existing study, eight Rosaceae species i.e., Pyrus communis, Prunus persica, Fragaria vesca, Pyrus bretschneideri, Prunus avium, Prunus mume, Rubus occidentalis, and Malus × domestica were evaluated. Here, we determined a total of 124 LYP genes from the underlined Rosaceae species. While eighteen of the genes were from Chinese white pear, named as PbrLYPs. According to the LYPs structural characteristics and their phylogenetic analysis, those genes were classified into eight groups (group LYK1, LYK2, LYK3, LYK4/5, LYM1/3, LYM2, NFP, and WAKL). Dispersed duplication and whole-genome duplication (WGD) were found to be the most contributing factors of LYP family expansion in the Rosaceae species. More than half of the duplicated PbrLYP gene pairs were dated back to the ancient WGD (~ 140 million years ago (MYA)), and PbrLYP genes have experienced long-term purifying selection. The transcriptomic results indicated that the PbrLYP genes expression was tissue-specific. Most PbrLYP genes showed differential expression in leaves under fungal pathogen infection with two of them located in the plasmalemma. CONCLUSION:A comprehensive analysis identified 124 LYP genes in eight Rosaceae species. Our findings have provided insights into the functions and characteristics of the Rosaceae LYP genes and a guide for the identification of other candidate LYPs for further genetic improvements for pathogen-resistance in higher plants.

译文

背景:含赖氨酸基序的蛋白质(LYP),作为模式识别受体,在生长,结节形成和对生物胁迫的响应中起着核心作用。中国白梨基因组(c山苏里)与其他七种蔷薇科的序列已被报道。尽管在这些水果作物中,关于LYP家族基因及其进化史仍然缺乏明确性。
结果:在现有研究中,评估了八种蔷薇科物种,即Pyrus communis,Prunus persica,Fragaria vesca,Pyrus bretschneideri,Prunus avium,Prunus mume,Rubus occidentalis和Malus×domestica。在这里,我们从下划线的蔷薇科物种中确定了总共124个LYP基因。其中有18个基因来自中国白梨,名为PbrLYPs。根据LYPs的结构特征和系统发育分析,将这些基因分为八类(LYK1,LYK2,LYK3,LYK4 / 5,LYM1 / 3,LYM2,NFP和WAKL)。分散复制和全基因组复制(WGD)被发现是蔷薇科物种LYP家族扩张的最主要因素。重复的PbrLYP基因对中有一半以上可追溯到古代WGD(约1.4亿年前(MYA)),并且PbrLYP基因经历了长期的纯化选择。转录组结果表明PbrLYP基因表达是组织特异性的。大多数PbrLYP基因在真菌病原体感染下在叶片中显示差异表达,其中两个位于浆膜中。
结论:综合分析鉴定了八种蔷薇科的124个LYP基因。我们的发现为酒渣鼻LYP基因的功能和特性提供了见识,并为其他候选LYP的鉴定提供了指导,以进一步遗传改良高等植物中的病原体抗性。

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