A study was conducted to determine the changes that occur to proteolysis and related genes due to age, protein, and energy intake in high-yield broiler breeder hens (Gallus gallus). Cobb 700 broiler breeders were randomly assigned to one of six diets in a 2×3 factorial fashion. Two levels of energy (390 and 450 kcal/day) and three levels of protein (22, 24, and 26 g CP/day) were utilized. Protein turnover was determined in the left pectoralis at 22, 26, 31 and 44 weeks. Relative mRNA expression of calpain 2 (CAPN2), proteasome C2 subunit (PSMA1), and F box protein 32 (FBXO32) were determined via RT-PCR at 20, 25, and 44 weeks. Contrasts indicate fractional synthesis rate (FSR) and FBXO32 increase to a maximum at 25-26 weeks and a decrease thereafter. A significant drop in PSMA1 and FBXO32 was observed between 25 and 44 weeks and matched the decrease observed in FBR. No differences were detected in the levels of fractional synthesis and degradation, or the expression of CAPN2, PSMA1, and FBXO32, due to protein or energy intake. In summary, protein turnover was upregulated during the transition into sexual maturity and decreased thereafter. The observed changes in degradation appeared to be mediated by the ubiquitin-proteasome pathway.

译文

:进行了一项研究,以确定高产肉鸡种鸡(鸡)因年龄,蛋白质和能量摄入而引起的蛋白水解和相关基因的变化。将Cobb 700肉鸡育种家以2×3阶乘方式随机分配到六种日粮中的一种。利用了两个水平的能量(390和450 kcal /天)和三个水平的蛋白质(22、24和26 g CP /天)。在22、26、31和44周时测定左胸大肌的蛋白质更新。通过RT-PCR在20、25和44周确定钙蛋白酶2(CAPN2),蛋白酶体C2亚基(PSMA1)和F盒蛋白32(FBXO32)的相对mRNA表达。对比表明分数合成速率(FSR)和FBXO32在25-26周时增加到最大值,然后降低。在25至44周之间,观察到PSMA1和FBXO32显着下降,并且与FBR中的下降相符。由于蛋白质或能量的摄入,未检测到部分合成和降解水平或CAPN2,PSMA1和FBXO32的表达水平差异。总之,在向性成熟过渡的过程中蛋白质周转率上调,此后下降。观察到的降解变化似乎是由泛素-蛋白酶体途径介导的。

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